User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09: Difference between revisions
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==WetLab== | ==WetLab== | ||
===Plasmid extraction pBBRmcS5. From DH5α.=== | ===Plasmid extraction pBBRmcS5. From DH5α.=== | ||
** | **First lane control | ||
**Plasmids 7 lanes. | **Plasmids 7 lanes. | ||
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===Plasmid digestion=== | ===Plasmid digestion=== | ||
*7 digestions with HindIII | *7 digestions with HindIII | ||
2μL 10X Buffer 2 NE | **2μL 10X Buffer 2 NE | ||
8μL H<sub>2</sub>0 | **8μL H<sub>2</sub>0 | ||
10μL DNA Plasmid stock | **10μL DNA Plasmid stock | ||
1μL Hind III. | **1μL Hind III. | ||
*Marks, | *Marks, | ||
**1 tube without DNA | **1 tube without DNA |
Revision as of 11:45, 1 July 2011
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WetLabPlasmid extraction pBBRmcS5. From DH5α.
Plasmid digestion
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