User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09: Difference between revisions

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==WetLab==
==WetLab==
===Plasmid extraction pBBRmcS5. From DH5α.===
===Plasmid extraction pBBRmcS5. From DH5α.===
**Firs lane control
**First lane control
**Plasmids 7 lanes.
**Plasmids 7 lanes.


Revision as of 11:45, 1 July 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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WetLab

Plasmid extraction pBBRmcS5. From DH5α.

    • First lane control
    • Plasmids 7 lanes.

Plasmid digestion

  • 7 digestions with HindIII
    • 2μL 10X Buffer 2 NE
    • 8μL H20
    • 10μL DNA Plasmid stock
    • 1μL Hind III.
  • Marks,
    • 1 tube without DNA
    • 1 tube without HindIII
    • 7 tubes with each plasmid stock.
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