User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/20: Difference between revisions

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==WetLab==
==WetLab==
*PCR from [http://partsregistry.org/Part:pSB1T3 pSB13T] Plate 1 A7.
*PCR from [http://partsregistry.org/Part:pSB1T3 pSB13T] Plate 1 A7.
**Ask to Uriel delimitations of PCR
**Phusion protocol
**PCR from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/19 | extracted plasmid]]
**PCR from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/19 | extracted plasmid]]
**Performed:
**Performed:

Revision as of 15:31, 26 May 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

WetLab

PCR‡ -R -F -DNA -Oligos 5μL DNA 10μL DNA
Forward 2 0 2 0 2 2
Reverse 0 2 2 0 2 2
dNTPs .4 .4 .4 .4 .4 .4
Buffer 4 4 4 4 4 4
Phusion .2 .2 .2 .2 .2 .2
DNA 10 10 0 10 5 10
H2O 3.4 3.4 11.4 5.4 6.4 1.4
    • ‡ μL Units
    • Phusion protocol. H2O Full up 20μL
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