User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/29: Difference between revisions

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(Autocreate 2011/05/29 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook)
 
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==Entry title==
==WetLab==
* Insert content here...
* Purification of digestion's products.
**I left over night digestion, trying to get the best digestion as possible.
**Inactivated enzymes at 65°C, 20 minutes.
**High Pure PCR Product Purification Kit.


*Electrophoresis gel of digestion
{| border="1"
!1
!2
!3
!4
!5
!6
!7
!8
|--
|Ladder 1Kb
|5μL ‡
|5μL †
|10μL †
|10μL ‡
|Control of Digestion
|pBBRmcS5†
|pBBRmcS5‡
|}
†[[User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/05/27 | Daniel´s extraction]]
‡[[User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28 |Fabricio´s extraction]]


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Revision as of 15:46, 29 May 2011

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WetLab

  • Purification of digestion's products.
    • I left over night digestion, trying to get the best digestion as possible.
    • Inactivated enzymes at 65°C, 20 minutes.
    • High Pure PCR Product Purification Kit.
  • Electrophoresis gel of digestion
1 2 3 4 5 6 7 8
Ladder 1Kb 5μL ‡ 5μL † 10μL † 10μL ‡ Control of Digestion pBBRmcS5† pBBRmcS5‡


† Daniel´s extraction

‡Fabricio´s extraction


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