User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/29

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WetLab

  • Purification of digestion's products.
    • I left over night digestion, trying to get the best digestion as possible.
    • Inactivated enzymes at 65°C, 20 minutes.
    • High Pure PCR Product Purification Kit.
  • Electrophoresis gel of digestion
1 2 3 4 5 6 7 8
Ladder 1Kb 5μL ‡ 5μL † 10μL † 10μL ‡ Control of Digestion pBBRmcS5† pBBRmcS5‡


Daniel´s extraction

Fabricio´s extraction



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