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 iGEM 2011
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WetLab
- Purification of digestion's products.
- I left over night digestion, trying to get the best digestion as possible.
- Inactivated enzymes at 65°C, 20 minutes.
- High Pure PCR Product Purification Kit.
- Electrophoresis gel of digestion
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| Ladder 1Kb
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5μL ‡
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5μL †
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10μL †
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10μL ‡
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Control of Digestion
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pBBRmcS5†
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pBBRmcS5‡
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† Daniel´s extraction
‡Fabricio´s extraction
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iGEM 2011
