User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/20: Difference between revisions
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*Primers (5 pM/μL) (new set because we are improving new protocol of standardization). | *Primers (5 pM/μL) (new set because we are improving new protocol of standardization). | ||
**Primer Forward= CGG GCC CCA TTA TTA TCA TGA CAT TAA CCT ATA AAA ATA GG | **Primer Forward= CGG GCC CCA TTA TTA TCA TGA CAT TAA CCT ATA AAA ATA GG | ||
***It includes GGGCCC. Site for ApaI. | |||
**Primer Reverse= CGA GCT CGC CTT TTG CTC ACA TCT TCT TTC C | **Primer Reverse= CGA GCT CGC CTT TTG CTC ACA TCT TCT TTC C | ||
***It includes GAGCTC. Site SacI. | |||
==PCR== | ==PCR== | ||
*DNA was obtained from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/19|extractions]] and diluted(2μL DNA in 98μL H<sub>2</sub>O) | *DNA was obtained from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/19|extractions]] and diluted(2μL DNA in 98μL H<sub>2</sub>O) | ||
Revision as of 11:09, 28 June 2011
 iGEM 2011
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Objectives
PCR
PCR amplification
Control PCR
Expectatives
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