User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/29: Difference between revisions
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* Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] and liquid for extraction. | * Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] and liquid for extraction. | ||
* See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| extractions]] of pUX19 cut with SmaI. | * See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| extractions]] of pUX19 cut with SmaI. | ||
* Digestion of pSB1AK3/BBa_B0015 with XbaI to see size. | |||
==Gel of SmaI restriction== | ==Gel of SmaI restriction== | ||
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Over night reaction. | |||
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Revision as of 11:05, 30 July 2011
 iGEM 2011
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Objectives
Gel of SmaI restrictionI expect 6.2Kbp~
Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction. ExtractionExtraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep.
I just need it to see barely concentration for digestion.
Digestion
Over night reaction.
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