User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/01: Difference between revisions
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Initialization step: 3:00min 95°C | |||
Denaturation step: 30seg 94°C | |||
Annealing step: 30seg 55°C | |||
Extension/elongation step: 40seg 72°C | |||
Final elongation 30seg 72°C | |||
Final hold: 3hrs 4°C | |||
==Gel of PCR and digestion== | |||
[[Image:PCR.Prom Km.B0015.1Aug2011.JPG|200px]] | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 22:21, 1 August 2011
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Objectives
Digestion
PCR for Kanamycin
Denaturation step: 30seg 94°C Annealing step: 30seg 55°C Extension/elongation step: 40seg 72°C Final elongation 30seg 72°C Final hold: 3hrs 4°C Gel of PCR and digestion
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