User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/03: Difference between revisions

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[[Image:3.Aug.2011.pSB1C3 1-3.B0015 3-6.JPG|130px]]
[[Image:3.Aug.2011.pSB1C3 1-3.B0015 3-6.JPG|130px]]


I will cut with XhoI pSB1C3 and pSB1AK3/BBa_B0015 I have previously [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/30|cut]]
I will cut with XhoI pSB1C3, for pSB1AK3/BBa_B0015 I have previously [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/30|cut]]




Revision as of 06:36, 4 August 2011

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Objectives

  • Transformation of Ligation Prom--Kanamycin + BBa_B0015(double ter) in pSB1AK3.
  • Plasmid extraction of pSB1C3(A3 plate 1 dist 2011) and pSB1AK3/BBa_B0015 (backbone and double ter).

Transformation

  • Was performed with yesterday´s ligation in DH5α E.Coli.

I hope kanamycin resistance, by backbone and I need a intesive search for false positives and I have control for it.

Method:Miguel´s Lab method.

Plasmid extraction

  • Miguel´s Lab method (miniprep)
  • Lane 1-3 pSB1C3
  • Lane 4-6 pSB1AK3/BBa_B0015.

I will cut with XhoI pSB1C3, for pSB1AK3/BBa_B0015 I have previously cut



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