User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/18: Difference between revisions
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(Autocreate 2011/08/18 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook) |
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== | =Objectives= | ||
* | * PCR of B0015. | ||
* PCR of pSB1T3. | |||
* See the in Gel. | |||
=Working= | |||
==PCR of B0015== | |||
The point of this PCR is get double terminator and insert it into pSB1T3 backbone. | |||
*Amplified with preffix and suffix primers. | |||
{| border="1" | |||
!Reactives | |||
!μL | |||
|-- | |||
|Buffer Taq Standard 10X | |||
|5 | |||
|-- | |||
|dNTPs 8mM | |||
|1.25 | |||
|-- | |||
|Oligo F/R | |||
| 2.5/2.5 | |||
|-- | |||
| DNA [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/17| B0015]] | |||
|1 | |||
|-- | |||
|H<sub>2</sub>O | |||
|37.75 | |||
|-- | |||
| Taq DNA | |||
| 1 | |||
|-- | |||
|Total | |||
| 50μL | |||
|} | |||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 10:38, 19 August 2011
 iGEM 2011
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Objectives
WorkingPCR of B0015The point of this PCR is get double terminator and insert it into pSB1T3 backbone.
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