User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/18: Difference between revisions
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==Purification of PCR product== | ==Purification of PCR product== | ||
Both were purified with Roche´s Kit for PCR purification | Both were purified with Roche´s Kit for PCR purification | ||
==Restriction== | |||
I performed restriction with EcoRI and PstI. | |||
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Poner esto RECUERDA!!! | |||
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Revision as of 11:55, 19 August 2011
 iGEM 2011
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Objectives
WorkingPCR of B0015The point of this PCR is get double terminator and insert it into pSB1T3 backbone.
Gel B0015
PCR of pSB1T3
All 2X. Two PCRs. Gel PCR of pSB1T3 backbone
As expected :) Both amplified. Purification of PCR productBoth were purified with Roche´s Kit for PCR purification RestrictionI performed restriction with EcoRI and PstI. Poner esto RECUERDA!!!
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