User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/18: Difference between revisions
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==PCR of pSB1T3== | ==PCR of pSB1T3== | ||
*PCR of pSB1T3/RFP, to amplify just | *PCR of pSB1T3/RFP, to amplify just backbone. | ||
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!Reagents | !Reagents |
Revision as of 16:48, 25 August 2011
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Objectives
WorkingPCR of B0015The point of this PCR is get double terminator and insert it into pSB1T3 backbone.
Gel B0015
PCR of pSB1T3
All 2X. Two PCRs. Gel PCR of pSB1T3 backbone
As expected :) Both amplified. Purification of PCR productBoth were purified with Roche´s Kit for PCR purification RestrictionI performed restriction with EcoRI and PstI. Poner esto RECUERDA!!!
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