User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/23: Difference between revisions
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=Objectives= | =Objectives= | ||
* Gel extraccion of [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/19]] | * Gel extraccion of [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/19| PCRs]] | ||
* PCR for Insert. Again! Low concentration in gel extraction. | |||
==Gel extraction== | ==Gel extraction== | ||
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I need to repeat the insert PCR and make a new Gel extraction for that biopart. | I need to repeat the insert PCR and make a new Gel extraction for that biopart. | ||
==PCR for inserts== | |||
{| border=1 | |||
!Reagents | |||
!PCR 1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 P1A7/2011] | |||
!PCR 2 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 P1A7/2011] | |||
!PCR 3 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 P1A7/2011](Control+) | |||
|-- | |||
|DNA | |||
|1μL | |||
|1μL | |||
|1μL | |||
|-- | |||
|Buffer 5x PhusionHF | |||
|10μL | |||
|10μL | |||
|10μL | |||
|-- | |||
|dNTPs(8mM) | |||
|5μL | |||
|5μL | |||
|5μL | |||
|-- | |||
|Oligo(Extnd_Pref_Fwd) | |||
|5μL | |||
|5μL | |||
|5μL‡ | |||
|-- | |||
|Oligo(Extnd_Suff_Rev) | |||
|5μL | |||
|5μL | |||
|5μL‡ | |||
|-- | |||
|Phusion | |||
|.5μL | |||
|.5μL | |||
|.5μL | |||
|-- | |||
|H<sub>2</sub>O | |||
|23.5μL | |||
|23.5μL | |||
|23.5μL | |||
|} | |||
‡Prefix_Fwd and Suffix_Reverse Primers. | |||
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Revision as of 16:59, 24 August 2011
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Objectives
Gel extraction
I need to repeat the insert PCR and make a new Gel extraction for that biopart.
PCR for inserts
‡Prefix_Fwd and Suffix_Reverse Primers.
|