User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/04: Difference between revisions
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We are trying to know what is happening with PCRs. | We are trying to know what is happening with PCRs. | ||
==PCR with different parameters== | |||
So far, we have amplified HydG and PFOR1, since now we are trying a new approach. For that we made a simply PCR, this is without GC buffer and DMSO. | |||
PCR is for HydEF1 and HydEF2 | |||
{|border=1|left | |||
|-- | |||
|55°C | |||
|60°C | |||
|-- | |||
|HydEF1 | |||
|HydEF2 | |||
|-- | |||
|HydEF1 | |||
|HydEF2 | |||
|} | |||
{| border=1 | |||
!Reagents | |||
!55°C | |||
!60°C | |||
|-- | |||
|Buffer HF Phusion | |||
|10μL | |||
|10μL | |||
|-- | |||
|dNTPs (10mM each) | |||
|4μL | |||
|4μL | |||
|-- | |||
|DNA diluited 10ng/μL | |||
| | |||
|} | |||
Revision as of 15:40, 5 September 2011
iGEM 2011 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||
Goal
Troubleshooting
The objectives of those changes are:
GelCompletely unsuccessful We are trying to know what is happening with PCRs.
PCR with different parametersSo far, we have amplified HydG and PFOR1, since now we are trying a new approach. For that we made a simply PCR, this is without GC buffer and DMSO. PCR is for HydEF1 and HydEF2
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