User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/04: Difference between revisions
From OpenWetWare
Line 27: | Line 27: | ||
==PCR with different parameters== | ==PCR with different parameters== | ||
So far, we have amplified HydG and PFOR1, since now we are trying a new approach. For that we made a simply PCR, this is without GC buffer and DMSO. | So far, we have amplified HydG and PFOR1, since now we are trying a new approach. For that we made a simply PCR, this is without GC buffer and DMSO. | ||
PCR is for HydEF1 and HydEF2 | PCR is for HydEF1 and HydEF2 ( new diluted plasmid with 10ng/μL ) | ||
{|border=1|left | {|border=1|left |
Revision as of 16:09, 5 September 2011
iGEM 2011 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||
Goal
Troubleshooting
The objectives of those changes are:
GelCompletely unsuccessful We are trying to know what is happening with PCRs.
PCR with different parametersSo far, we have amplified HydG and PFOR1, since now we are trying a new approach. For that we made a simply PCR, this is without GC buffer and DMSO. PCR is for HydEF1 and HydEF2 ( new diluted plasmid with 10ng/μL )
|