User:Jose Fabricio Lopez/Notebook/Logbook/2011/09/08: Difference between revisions

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Revision as of 14:35, 12 September 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Goals

  • PCR for TerB0015-Backbone and PCR for HydEF1.

PCRs

PCR for TerB0015-Backbone
Reagents μL MegaMix
H2O 24.5 50
5X Phusion Buffer HF 10 20.1
10mM dNTPs 1 each 2 each
Primer F 5 10.2
Primer R 5 10.2
DNA 1 2
Phusion .5 NA
PCR for TerB0015-Backbone
Reagents μL MegaMix
H2O 23 46.5
5X Phusion Buffer HF 10 20.1
10mM dNTPs 1 each 2 each
Primer F 5 NA
Primer R 5 NA
DNA 1 2
DMSO 1.5 3
Phusion .5 NA
  • Lane1 Ladder
  • Lane2 Ter1-Backbone
  • Lane3 Ter2-Backbone
  • Lane4 HydEF 1
  • Lane5 HydEF 2



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