User:Joytafty/Notebook/Effects of EphA2 and CXCR3 KD in hMVECs/2012/04/09: Difference between revisions
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* Wipe off sample pedestal and blank the device with 2 uL of buffer that sample is in (from this protocol, it is DEPC-treated water). | * Wipe off sample pedestal and blank the device with 2 uL of buffer that sample is in (from this protocol, it is DEPC-treated water). | ||
* Wipe off the pedestal and pipette 2 uL of the sample on to measure. Note: Read only a volume of sample once. If make an additional read on the sample, it will change. To get replicate readings, load another 2 uL of the sample on. | * Wipe off the pedestal and pipette 2 uL of the sample on to measure. Note: Read only a volume of sample once. If make an additional read on the sample, it will change. To get replicate readings, load another 2 uL of the sample on. | ||
==Result== | |||
{| | |||
! align="left"| Sample | |||
! [RNA] | |||
! [230/280] | |||
! [260/280] | |||
|- | |||
| align="left"| 24h1 75 nM CXCR3 RNAi Read1 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 75 nM CXCR3 RNAi Read2 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 50 nM CXCR3 RNAi Read1 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 50 nM CXCR3 RNAi Read2 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 25 nM CXCR3 RNAi Read1 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 25 nM CXCR3 RNAi Read2 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 10 nM CXCR3 RNAi Read1 | |||
| | |||
| | |||
| | |||
|- | |||
| align="left"| 24h1 10 nM CXCR3 RNAi Read2 | |||
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|- | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 15:11, 9 April 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||
RNA extraction of 2012/03/30 samplesGoal:
Background:
Materials:
Procedure: Preparation
KEEP ON ICE FROM THIS POINT ON
How to run Nanodrop:
Result
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