User:Justinhlo: Difference between revisions
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===What were the:=== | ===What were the:=== | ||
==== | ====Densities of your two samples?==== | ||
LB: 2.77x10<sup>9</sup> cells/mL | LB: 2.77x10<sup>9</sup> cells/mL | ||
Line 39: | Line 39: | ||
SOC: 6905 units | SOC: 6905 units | ||
==== | ====Concentrations of total RNA that you isolated from the two samples?==== | ||
LB: 158.4 μg/mL | LB: 158.4 μg/mL |
Revision as of 12:03, 20 April 2006
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Record for experiment addressing Coli-roid operating conditions
Lab section
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((T/R)) | W/F |
Team color
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Green | ((Purple)) | Blue | Red | Pink | Yellow |
Describe the experimental variations you are comparing.
We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium).
What were the:
Densities of your two samples?
LB: 2.77x109 cells/mL SOC: 1.53x109 cells/mL
β-gal values measured for the two samples?
LB: 7995 units SOC: 6905 units
Concentrations of total RNA that you isolated from the two samples?
LB: 158.4 μg/mL SOC: 46.4 μg/mL
Method of cDNA synthesis
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((Epicenter)) | Invitrogen |
Primer pair used
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5' end of LacZ gene | Middle of LacZ gene | 3' end of LacZ |