User:Justinhlo

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Hello, everyone!  I'm currently taking [[BE.109]] as well as [[BE.180]] as part of my spring 2006 curriculumI am also doing research in [[Bevin_Engelward | Bevin Engelward]]'s lab.
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Instructions: Copy this page to your user page and fill it out on the wiki for submission.   
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==Education==
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==Record for experiment addressing Coli-roid operating conditions==
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*Chapel Hill High School (2000-2004)
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*Massachusetts Institute of Technology (2004-[2008]), majoring in BE (and music?)
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==Links==
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===Lab section===
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*My personal webpage (under construction, eww): [http://web.mit.edu/jhlo/www/ Symphony of the Winds]
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''Choose one''
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*My lab's webpage: [http://web.mit.edu/bevin/www/ Engelward Lab]
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{|
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| style="width:100px" | <b>((T/R))</b>
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| style="width:100px" | W/F
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|}
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===Team color===
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''Choose one''
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{|
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| style="width:100px" | Green
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| style="width:100px" | <b>((Purple))</b>
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| style="width:100px" | Blue
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| style="width:100px" | Red
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| style="width:100px" | Pink
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| style="width:100px" | Yellow
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|}
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===Describe the experimental variations you are comparing.===
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We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium). 
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===What were the:===
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====density of your two samples?====
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LB: 2.77x10<sup>9</sup> cells/mL
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SOC: 1.53x10<sup>9</sup> cells/mL
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====β-gal values measured for the two samples?====
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LB: 7995 units
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SOC: 6905 units
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====concentrations of total RNA that you isolated from the two samples?====
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LB: 158.4 μg/mL
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SOC: 46.4 μg/mL
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====Method of cDNA synthesis====
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''Choose one''
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{|
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| style="width:100px" | <b>((Epicenter))</b>
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| style="width:100px" | Invitrogen
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|}
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===Primer pair used===
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''Choose one''
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{|
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| style="width:200px" | 5' end of LacZ gene
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| style="width:200px" | Middle of LacZ gene
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| style="width:200px" | 3' end of LacZ
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|}
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===Wells for your samples:===
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[[Image:Macintosh HD-Users-nkuldell-Desktop-RTPCRplate.png| RT-PCR 96 well plate]]

Revision as of 14:02, 20 April 2006

Instructions: Copy this page to your user page and fill it out on the wiki for submission.

Contents

Record for experiment addressing Coli-roid operating conditions

Lab section

Choose one

((T/R)) W/F

Team color

Choose one

Green ((Purple)) Blue Red Pink Yellow

Describe the experimental variations you are comparing.

We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium).

What were the:

density of your two samples?

LB: 2.77x109 cells/mL SOC: 1.53x109 cells/mL

β-gal values measured for the two samples?

LB: 7995 units SOC: 6905 units

concentrations of total RNA that you isolated from the two samples?

LB: 158.4 μg/mL SOC: 46.4 μg/mL

Method of cDNA synthesis

Choose one

((Epicenter)) Invitrogen

Primer pair used

Choose one

5' end of LacZ gene Middle of LacZ gene 3' end of LacZ

Wells for your samples:

RT-PCR 96 well plate

Personal tools