# User:Justinhlo

(Difference between revisions)
 Revision as of 09:24, 9 February 2006 (view source)← Previous diff Revision as of 15:02, 20 April 2006 (view source)Next diff → Line 1: Line 1: - Hello, everyone!  I'm currently taking [[BE.109]] as well as [[BE.180]] as part of my spring 2006 curriculum.  I am also doing research in [[Bevin_Engelward | Bevin Engelward]]'s lab. + Instructions: Copy this page to your user page and fill it out on the wiki for submission. - ==Education== + ==Record for experiment addressing Coli-roid operating conditions== - *Chapel Hill High School (2000-2004) + - *Massachusetts Institute of Technology (2004-[2008]), majoring in BE (and music?) + - ==Links== + ===Lab section=== - *My personal webpage (under construction, eww): [http://web.mit.edu/jhlo/www/ Symphony of the Winds] + ''Choose one'' - *My lab's webpage: [http://web.mit.edu/bevin/www/ Engelward Lab] + + {| + | style="width:100px" | ((T/R)) + | style="width:100px" | W/F + |} + + ===Team color=== + ''Choose one'' + + {| + | style="width:100px" | Green + | style="width:100px" | ((Purple)) + | style="width:100px" | Blue + | style="width:100px" | Red + | style="width:100px" | Pink + | style="width:100px" | Yellow + |} + + ===Describe the experimental variations you are comparing.=== + + We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium). + + ===What were the:=== + + ====density of your two samples?==== + + LB: 2.77x109 cells/mL + SOC: 1.53x109 cells/mL + + ====β-gal values measured for the two samples?==== + + LB: 7995 units + SOC: 6905 units + + ====concentrations of total RNA that you isolated from the two samples?==== + + LB: 158.4 μg/mL + SOC: 46.4 μg/mL + + ====Method of cDNA synthesis==== + ''Choose one'' + + {| + | style="width:100px" | ((Epicenter)) + | style="width:100px" | Invitrogen + |} + + ===Primer pair used=== + ''Choose one'' + + {| + | style="width:200px" | 5' end of LacZ gene + | style="width:200px" | Middle of LacZ gene + | style="width:200px" | 3' end of LacZ + |} + + ===Wells for your samples:=== + + [[Image:Macintosh HD-Users-nkuldell-Desktop-RTPCRplate.png| RT-PCR 96 well plate]]

## Revision as of 15:02, 20 April 2006

Instructions: Copy this page to your user page and fill it out on the wiki for submission.

## Record for experiment addressing Coli-roid operating conditions

### Lab section

Choose one

 ((T/R)) W/F

### Team color

Choose one

 Green ((Purple)) Blue Red Pink Yellow

### Describe the experimental variations you are comparing.

We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium).

### What were the:

#### density of your two samples?

LB: 2.77x109 cells/mL SOC: 1.53x109 cells/mL

#### β-gal values measured for the two samples?

LB: 7995 units SOC: 6905 units

#### concentrations of total RNA that you isolated from the two samples?

LB: 158.4 μg/mL SOC: 46.4 μg/mL

#### Method of cDNA synthesis

Choose one

 ((Epicenter)) Invitrogen

### Primer pair used

Choose one

 5' end of LacZ gene Middle of LacZ gene 3' end of LacZ