User:Justinhlo

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===What were the:===
===What were the:===
-
====density of your two samples?====
+
====Densities of your two samples?====
LB: 2.77x10<sup>9</sup> cells/mL
LB: 2.77x10<sup>9</sup> cells/mL
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SOC: 6905 units
SOC: 6905 units
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====concentrations of total RNA that you isolated from the two samples?====
+
====Concentrations of total RNA that you isolated from the two samples?====
LB: 158.4 μg/mL
LB: 158.4 μg/mL

Revision as of 14:03, 20 April 2006

Instructions: Copy this page to your user page and fill it out on the wiki for submission.

Contents

Record for experiment addressing Coli-roid operating conditions

Lab section

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((T/R)) W/F

Team color

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Green ((Purple)) Blue Red Pink Yellow

Describe the experimental variations you are comparing.

We are comparing the type of medium used to grow the E. Coli: LB vs. SOC (rich medium).

What were the:

Densities of your two samples?

LB: 2.77x109 cells/mL SOC: 1.53x109 cells/mL

β-gal values measured for the two samples?

LB: 7995 units SOC: 6905 units

Concentrations of total RNA that you isolated from the two samples?

LB: 158.4 μg/mL SOC: 46.4 μg/mL

Method of cDNA synthesis

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((Epicenter)) Invitrogen

Primer pair used

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5' end of LacZ gene Middle of LacZ gene 3' end of LacZ

Wells for your samples:

RT-PCR 96 well plate

Personal tools