User:Kara Tsukashima/Notebook/Yogurt Lactobacillus RFLP identification/2013/04/29

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Current revision (22:49, 29 April 2013) (view source)
(Observation of growth)
 
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==Observation of growth==
==Observation of growth==
It was decided that, given the minimal growth, it would be best to leave the plates in the incubator until Saturday to enable further growth.
It was decided that, given the minimal growth, it would be best to leave the plates in the incubator until Saturday to enable further growth.
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==Future plans==
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I'll pick colonies off of the isolation plates on 4 May 2013 (Saturday) and incubate them for a further week, as this aerobic LB agar environment is hardly the ideal.
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Thus on 11 May 2013 (next Saturday) I should be able to get here early and do the PCR, digest and gel throughout the day. Primers will need to be ordered.
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 +
Again, the plan is to more or less follow the procedure of [http://link.springer.com/article/10.1023%2FB%3AWIBI.0000043151.09366.d7 this] 2004 paper for the amplification and digest.

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Observation of growth

It was decided that, given the minimal growth, it would be best to leave the plates in the incubator until Saturday to enable further growth.


Future plans

I'll pick colonies off of the isolation plates on 4 May 2013 (Saturday) and incubate them for a further week, as this aerobic LB agar environment is hardly the ideal.

Thus on 11 May 2013 (next Saturday) I should be able to get here early and do the PCR, digest and gel throughout the day. Primers will need to be ordered.

Again, the plan is to more or less follow the procedure of this 2004 paper for the amplification and digest.



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