User:Karlena L. Brown/Notebook/PVOH Research/2012/10/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 102: Line 102:
# Place container with the solution on a stir plate and stir solution for ~20 minutes or until all solids dissipate into solution
# Place container with the solution on a stir plate and stir solution for ~20 minutes or until all solids dissipate into solution
# Add 20mL of concentrated H<sub>2</sub>SO<sub>4</sub> to solution and continue stirring for ~10 minutes more
# Add 20mL of concentrated H<sub>2</sub>SO<sub>4</sub> to solution and continue stirring for ~10 minutes more
==Crosslinking Porphyrin Films==
'''Follow same protocal regarding crosslinking glutaldehyde films; however, pay attention to the two PVOH films that will not be placed in the fixant solution during the crosslinking procedure.'''


==Notes==
==Notes==

Revision as of 21:22, 23 November 2012

PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

OBJECTIVES

  • Prepare new CuSO4 standards for Atomic Absorption
  • Re-examine NaMT-CuSO4 exchanged filtrate solutions using Atomic Absorption
  • Crosslink all porphyrin films prepared
  • Prepare new crosslinking fixant solutions

New CuSO4 Dilution Standards for Atomic Absorption

  • Solution 0.1 ppm CuSO4
  (20 ppm CuSO4)V1 = (0.1 ppm CuSO4)(12mL)
   V1 = 0.6mL of 20 ppm CuSO4
  • Solution 0.5 ppm CuSO4 requires
  (20 ppm CuSO4)V1 = (0.5 ppm CuSO4)(12mL)
   V1 = 1.2mL of 20 ppm CuSO4
  • Solution 1 ppm CuSO4 requires
  (20 ppm CuSO4)V1 = (1 ppm CuSO4)(12mL)
   V1 = 3mL of 20 ppm CuSO4
  • Solution 2 ppm CuSO4 requires
  (20 ppm CuSO4)V1 = (2 ppm CuSO4)(12mL)
   V1 = 6mL of 20 ppm CuSO4
  • Solution 3 ppm CuSO4 requires
  (20 ppm CuSO4)V1 = (3 ppm CuSO4)(12mL)
   V1 = 9mL of 20 ppm CuSO4

Using the following concentrations(shown above), a calibration curve was prepared.

Atomic Absorption NaMT-CuSO4 Exchanged Filtrate Solutions

' Concentration [ppm] Abs. [ ] BG '
0.01 -0.00600 0.00200
0.05 -0.01160 0.00540
1 -0.00920 0.00150
2 0.11710 0.00470
3 0.20330 0.00330
Given Conc. [ppm] Abs. [ ] BG Conc. [ppm]
1 min NaMT-CuSO4 7.4 0.37690 0.00430 5.63990
10 0.47600 0.00620 7.01910
5 min NaMT-CuSO4 7.4 0.38080 0.00350 5.69420
10 0.48150 0.00600 7.09560
10 min NaMT-CuSO4 7.4 0.29540 0.00370 4.50580
10 0.37640 0.00480 5.63300
1 min 100%CEC NaMT-CuSO4 7.4 0.48030 0.00680 7.07890
10 0.61880 0.00950 9.00630
5 min 100%CEC NaMT-CuSO4 7.4 0.46120 0.00640 6.81310
10 0.62080 0.00790 9.03420
10 min 100%CEC NaMT-CuSO4 7.4 0.43300 0.00400 6.42070
10 0.62960 0.00480 9.15660
1 min 50%CEC NaMT-CuSO4 7.4 0.47470 0.00530 7.00100
10 0.62050 0.00490 9.03000
5 min 50%CEC NaMT-CuSO4 7.4 0.46110 0.00310 6.81170
10 0.60940 0.00580 8.87550
10 min 50%CEC NaMT-CuSO4 7.4 0.46800 0.00390 6.90770
10 0.59620 0.00440 8.69180


Fixant Solution Preparation

  1. Weigh out ~200g Na2SO4
 (Actual Na2SO4 Mass: 199.9953g)
  1. Add the Na2SO4 solids to a 1000mL closed cap plastic bottle
  2. Next, add ~1000mL of H2O to the bottle along with large magnetic stir bar
  3. Place container with the solution on a stir plate and stir solution for ~20 minutes or until all solids dissipate into solution
  4. Add 20mL of concentrated H2SO4 to solution and continue stirring for ~10 minutes more

Crosslinking Porphyrin Films

Follow same protocal regarding crosslinking glutaldehyde films; however, pay attention to the two PVOH films that will not be placed in the fixant solution during the crosslinking procedure.

Notes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karlena_L._Brown/Notebook/PVOH_Research/2012/10/19&oldid=658296"