User:Karlena L. Brown/Notebook/PVOH Research/2012/10/24: Difference between revisions

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==100mM Salt Solution Calculations==
==100mM Salt Solution Calculations==
   
   
* '''100mM Fe<sub>2</sub>SO<sub>4</sub> Solution'''
* '''100mM Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> Solution'''
   '''MW Fe<sub>2</sub>SO<sub>4</sub>: 278.91 g/mol'''
   '''MW Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>: 278.91 g/mol'''
   100mM Fe<sub>2</sub>SO<sub>4</sub> × (100mL H<sub>2</sub>O/1000mL H<sub>2</sub>O) × (278.91g Fe<sub>2</sub>SO<sub>4</sub>/1 mol Fe<sub>2</sub>SO<sub>4</sub>) = 2.7891g Fe<sub>2</sub>SO<sub>4</sub>
   100mM Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> × (100mL H<sub>2</sub>O/1000mL H<sub>2</sub>O) × (278.91g Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>/1 mol Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>) = 2.7891g Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>
    
    
* '''100mM Al<sub>3</sub>SO<sub>4</sub> Solution'''
* '''100mM (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub> Solution'''
   '''MW Al<sub>3</sub>SO<sub>4</sub>: 356.37 g/mol
   '''MW (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub>: 356.37 g/mol
   100mM Al<sub>3</sub>SO<sub>4</sub> × (100mL H<sub>2</sub>O/1000mL H<sub>2</sub>O) × (656.37g Al<sub>3</sub>SO<sub>4</sub>/1 mol Al<sub>3</sub>SO<sub>4</sub>) = 6.5637g Al<sub>3</sub>SO<sub>4</sub>
   100mM (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub> × (100mL H<sub>2</sub>O/1000mL H<sub>2</sub>O) × (656.37g (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub>/1 mol (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub>) = 6.5637g (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub>
    
    
* '''100mM FeSO<sub>4</sub> Solution'''
* '''100mM FeSO<sub>4</sub> Solution'''
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| align="center" style="background:#f0f0f0;"|'''Total Concentration(mM)'''
| align="center" style="background:#f0f0f0;"|'''Total Concentration(mM)'''
|-
|-
| Fe<sub>2</sub>SO<sub>4</sub>||2.7891||2.8799||100||100
| Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>||2.7891||2.8799||100||100
|-
|-
| Al<sub>3</sub>SO<sub>4</sub>||6.5637||6.56314||100||100
| (Al<sub>3</sub>SO<sub>4</sub>)<sub>3</sub>||6.5637||6.56314||100||100
|-
|-
| FeSO<sub>4</sub>||1.3901||1.3904||100||100
| FeSO<sub>4</sub>||1.3901||1.3904||100||100

Revision as of 00:12, 24 November 2012

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OBJECTIVES

  • Make porphyrin solutions with varied SO42- salts
  • Take UV-Vis of porphyrin solutions with varied SO42- salts

Porphyrin Salt Selection and Preparations

SO42- salts used:Fe2(SO4)3, Al3(SO4)3, FeSO4,& Na2SO4

Preparing 100mM Stock Solutions For Each Salt :

  1. Obtain four clean 100mL volumetric flasks with stoppers
  2. Measure out calculated amounts of each salt to establish 100mM concentration
  3. Add varied salt amounts to each volumetric flask (one salt per flask)
  4. Fill each volumetric flask ½ full with HPLC grade H2O
  5. Swirl each flask thoroughly for ~ 5 minutes to begin the dissolving process
  6. Finish adding HPLC grade H2O to each volumetric flask to line

(Ensure that the volumetric calibration line is not surpassed or solution must be remade.)

100mM Salt Solution Calculations

  • 100mM Fe2(SO4)3 Solution
  MW Fe2(SO4)3: 278.91 g/mol
  100mM Fe2(SO4)3 × (100mL H2O/1000mL H2O) × (278.91g Fe2(SO4)3/1 mol Fe2(SO4)3) = 2.7891g Fe2(SO4)3
  • 100mM (Al3SO4)3 Solution
  MW (Al3SO4)3: 356.37 g/mol
  100mM (Al3SO4)3 × (100mL H2O/1000mL H2O) × (656.37g (Al3SO4)3/1 mol (Al3SO4)3) = 6.5637g (Al3SO4)3
  • 100mM FeSO4 Solution
  MW FeSO4: 278.01 g/mol
  100mM FeSO4 × (50mL H2O/1000mL H2O) × (278.01g FeSO4/1 mol FeSO4) = 1.3901g FeSO4
  • 100mM Na2SO4 Solution
  MW Na2SO4: 142.04 g/mol
  100mM Na2SO4 × (100mL H2O/1000mL H2O) × (142.04g Na2SO4/1 mol Na2SO4Na2SO4) = 1.4204g Na2SO4
SO42- Salts Selected Salt Mass Needed (g) Actual Salt Mass (g) HPLC Grade H2O Added (mL) Total Concentration(mM)
Fe2(SO4)3 2.7891 2.8799 100 100
(Al3SO4)3 6.5637 6.56314 100 100
FeSO4 1.3901 1.3904 100 100
Na2SO4 1.4204 1.4200 100 100

UV-Vis Spectrum of Porphyrin with Salts

A specific porphyrin solution (944.5nM) was selected for exemplary UV - Vis analysis pertaining to each salt. Furthermore, 0.1mL each salt was combined with this particular porphyrin solution.


Notes


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