User:Karlena L. Brown/Notebook/PVOH Research/2012/10/26: Difference between revisions
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==OBJECTIVES== | ==OBJECTIVES== | ||
* Make porphyrin solutions with varied SO<sub>4</sub><sup>2-</sup> salts | |||
* Take UV-Vis of varied porphyrin solutions with diluted varied SO<sub>4</sub><sup>2-</sup> salts | |||
==Porphyrin Salt Selection and Preparations 2== | ==Porphyrin Salt Selection and Preparations 2== | ||
SO<sub>4</sub><sup>2-</sup> salts used:'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>''' | |||
'''Preparing 10mM Stock Solutions For Each Salt :''' | |||
# Obtain four clean 100mL volumetric flasks with stoppers | |||
# Measure out calculated amounts of each salt to establish 10mM concentration | |||
# Add varied salt amounts to each volumetric flask (one salt per flask) | |||
# Fill each volumetric flask ½ full with HPLC grade H<sub>2</sub>O | |||
# Swirl each flask thoroughly for ~ 5 minutes to begin the dissolving process | |||
# Finish adding HPLC grade H<sub>2</sub>O to each volumetric flask to line | |||
'''(Ensure that the volumetric calibration line is not surpassed or solution must be remade.)''' | |||
==10mM Salt Solution Calculations== | |||
* '''10mM Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> Solution''' | |||
'''MW Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>: 278.91 g/mol''' | |||
10mM Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> × (100mL H<sub>2</sub>O / 1000mL H<sub>2</sub>O) × (278.91g Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> / 1 mol Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>) = 0.2789g Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> | |||
* '''10mM Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> Solution''' | |||
'''MW Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>: 356.37 g/mol | |||
10mM Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> × (100mL H<sub>2</sub>O / 1000mL H<sub>2</sub>O) × (656.37g Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> / 1 mol Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>) = 0.6564g Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> | |||
* '''10mM FeSO<sub>4</sub> Solution''' | |||
'''MW FeSO<sub>4</sub>: 278.01 g/mol''' | |||
10mM FeSO<sub>4</sub> × (50mL H<sub>2</sub>O / 1000mL H<sub>2</sub>O) × (278.01g FeSO<sub>4</sub> / 1 mol FeSO<sub>4</sub>) = 0.1390g FeSO<sub>4</sub> | |||
* '''10mM Na<sub>2</sub>SO<sub>4</sub> Solution''' | |||
'''MW Na<sub>2</sub>SO<sub>4</sub>: 142.04 g/mol''' | |||
10mM Na<sub>2</sub>SO<sub>4</sub> × (100mL H<sub>2</sub>O / 1000mL H<sub>2</sub>O) × (142.04g Na<sub>2</sub>SO<sub>4</sub> / 1 mol Na<sub>2</sub>SO<sub>4</sub>) = 0.1420g Na<sub>2</sub>SO<sub>4</sub> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''SO<sub>4</sub><sup>2-</sup> Salts Selected''' | |||
| align="center" style="background:#f0f0f0;"|'''Salt Mass Needed (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Actual Salt Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''HPLC Grade H<sub>2</sub>O Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Concentration(mM)''' | |||
|- | |||
| Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>||0.2879||0.3100||100||10 | |||
|- | |||
| Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>||0.6564||0.6556||100||10 | |||
|- | |||
| FeSO<sub>4</sub>||0.1390||0.1394||100||10 | |||
|- | |||
| Na<sub>2</sub>SO<sub>4</sub>||0.1420||0.1464||100||10 | |||
|} | |||
==UV-Vis Spectrum of Porphyrin with Salts 2== | ==UV-Vis Spectrum of Porphyrin with Salts 2== | ||
[[Image:9.445_uM_Porphine.JPG]] | |||
<br> | |||
[[Image:31.48_uM_Porphine.JPG]] | |||
<br> | |||
==Notes== | ==Notes== | ||
* The Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> 10mM solution was very opaque and cloudy with some precipitate slightly falling out of solution. Before using the Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> solution, it had to be swirled to ensure that all precipitate was in solution. | |||
* Also for the FeSO<sub>4</sub> 10mM salt solution, it was prepared in a 50mL volumetric flask instead of a 100mL volumetric flask. | |||
* Also for the 10mM salt solutions of Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> and Na<sub>2</sub>SO<sub>4</sub>, these solutions were actually prepared through the process of dilution in conical test tubes. 1mL of the 100mM salt solution for each substance was taken and diluted with 9mL of HPLC grade H<sub>2</sub>O to produce the 10mM salt solution of each substance. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 11:53, 30 November 2012
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OBJECTIVES
Porphyrin Salt Selection and Preparations 2SO42- salts used:Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4 Preparing 10mM Stock Solutions For Each Salt :
(Ensure that the volumetric calibration line is not surpassed or solution must be remade.) 10mM Salt Solution Calculations
MW Fe2(SO4)3: 278.91 g/mol 10mM Fe2(SO4)3 × (100mL H2O / 1000mL H2O) × (278.91g Fe2(SO4)3 / 1 mol Fe2(SO4)3) = 0.2789g Fe2(SO4)3
MW Al3(SO4)3: 356.37 g/mol 10mM Al3(SO4)3 × (100mL H2O / 1000mL H2O) × (656.37g Al3(SO4)3 / 1 mol Al3(SO4)3) = 0.6564g Al3(SO4)3
MW FeSO4: 278.01 g/mol 10mM FeSO4 × (50mL H2O / 1000mL H2O) × (278.01g FeSO4 / 1 mol FeSO4) = 0.1390g FeSO4
MW Na2SO4: 142.04 g/mol 10mM Na2SO4 × (100mL H2O / 1000mL H2O) × (142.04g Na2SO4 / 1 mol Na2SO4) = 0.1420g Na2SO4
UV-Vis Spectrum of Porphyrin with Salts 2Notes
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