User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07
From OpenWetWare
< User:Karlena L. Brown | Notebook | PVOH Research | 2012 | 11(Difference between revisions)
(→OBJECTIVES) |
Current revision (16:42, 30 November 2012) (view source) (→UV-Vis Limit of Detection For Salts) |
||
| (7 intermediate revisions not shown.) | |||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==OBJECTIVES== | ==OBJECTIVES== | ||
| - | * Make porphyrin solution with varied SO<sub>4</sub>< | + | * Make porphyrin solution with varied SO<sub>4</sub><sup>2-</sup> salts to uncover UV-Vis limit of detection (LOD) |
| - | * Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub>< | + | * Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub><sup>2-</sup> salts |
==UV-Vis Limit of Detection For Salts== | ==UV-Vis Limit of Detection For Salts== | ||
| - | + | '''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED''' | |
| + | To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared. | ||
| + | '''Solution of 1.89 μM porphyrin''' | ||
| + | (94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL) | ||
| + | V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin | ||
| + | |||
| + | {| {{table}} | ||
| + | | align="center" style="background:#f0f0f0;"|'''Porphyrine''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub> concentration''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub> concentration''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Volume of Water Added [mL]''' | ||
| + | |- | ||
| + | | 1.89 µM||1 mM||1 mM||1 mM||1 mM||9.9 | ||
| + | |- | ||
| + | | 1.89 µM||2 mM||2 mM||2 mM||2 mM||9.8 | ||
| + | |- | ||
| + | | 1.89 µM||5 mM||5 mM||5 mM||5 mM||9.5 | ||
| + | |- | ||
| + | | 1.89 µM||10 mM||10 mM||10 mM||10 mM||9 | ||
| + | |} | ||
| + | |||
| + | ==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations== | ||
| + | [[Image:Al2%28SO4%293.jpg]] | ||
| + | <br><br> | ||
| + | [[Image:Fe2%28SO4%293.jpg]] | ||
| + | <br><br> | ||
| + | [[Image:FeSO4.jpg]] | ||
| + | <br><br> | ||
| + | [[Image:Na2SO4.jpg]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Current revision
 PVOH Research
|  Main project page Previous entry Next entry
| ||||||||||||||||||||||||||||||
OBJECTIVES
UV-Vis Limit of Detection For SaltsSTOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED To find the limit of detection for the 10mM salts (Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared. Solution of 1.89 μM porphyrin (94.45 μM)(V1)=(1.89 μM)(10mL) V1 = 0.2mL of 94.45 μM porphyrin
UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations
| |||||||||||||||||||||||||||||||
