User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07: Difference between revisions

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==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
 
[[Image:Al2%28SO4%293.jpg]]
 
<br><br>
[[Image:Fe2%28SO4%293.jpg]]
<br><br>
[[Image:FeSO4.jpg]]
<br><br>
[[Image:Na2SO4.jpg]]


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Revision as of 11:43, 30 November 2012

PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin 

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9
1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8
1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5
1.89 µM 10 mM 10 mM 10 mM 10 mM 9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations








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