User:Karlena L. Brown/Notebook/PVOH Research/2013/01/30: Difference between revisions
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# The next day, remove the hydrogels in the freezer in order that the substances can thaw for ~ 24 hours | # The next day, remove the hydrogels in the freezer in order that the substances can thaw for ~ 24 hours | ||
# Continue the crosslinking freezing and thawing method for ~ 3 days | # Continue the crosslinking freezing and thawing method for ~ 3 days | ||
'''(Repeat entire process again when preparing hydrogels while using PVOH MW 130K samples)''' | |||
==50:50 PVOH Clay Hydrogel Preparations== | ==50:50 PVOH Clay Hydrogel Preparations== | ||
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# The next day, remove the hydrogels in the freezer in order that the substances can thaw for ~ 24 hours | # The next day, remove the hydrogels in the freezer in order that the substances can thaw for ~ 24 hours | ||
# Continue the crosslinking freezing and thawing method for ~ 3 days | # Continue the crosslinking freezing and thawing method for ~ 3 days | ||
'''(Repeat entire process again when preparing hydrogels while using PVOH MW 130K samples)''' | |||
==90:10 Rhodamine 6G Dye Ratio Solution Preparation== | ==90:10 Rhodamine 6G Dye Ratio Solution Preparation== | ||
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# Swirl periodically while filling flask with DMSO to the calibrated line | # Swirl periodically while filling flask with DMSO to the calibrated line | ||
'''90:10 Rhodamine 6G | '''90:10 Rhodamine 6G Dye Ratio Calculations''' | ||
(0.900g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0655mL | (0.900g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0655mL | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 6mL = (0.0655mL)M<sub>2</sub> M<sub>2</sub> = 92μM (RG6) | 1μM (RG6)x 6mL = (0.0655mL)M<sub>2</sub> M<sub>2</sub> = 92μM (RG6) | ||
92μM (RG6)(1x10<sup>-6</sup>M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.002g (RG6) | 92μM (RG6)(1x10<sup>-6</sup>M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.002g (RG6) | ||
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'''50:50 Rhodamine 6G Dye Ratio Calculations''' | '''50:50 Rhodamine 6G Dye Ratio Calculations''' | ||
(0.500g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0364mL | (0.500g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0364mL | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 6mL = (0.0364mL)M<sub>2</sub> M<sub>2</sub> = 165μM | 1μM (RG6)x 6mL = (0.0364mL)M<sub>2</sub> M<sub>2</sub> = 165μM | ||
165μM (RG6)(1x10<sup>-6</sup>M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.004g (RG6) | 165μM (RG6)(1x10<sup>-6</sup>M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.004g (RG6) | ||
==Important DMSO and Rhodamine 6G Safety== | ==Important DMSO and Rhodamine 6G Safety== | ||
* Dimethyl Sulfoxide is toxic colorless liquid (78. | * Dimethyl Sulfoxide is toxic colorless liquid '''(MW: 78.13 g/mole)''' | ||
* Avoid all dimethyl sulfoxide skin contact by wearing gloves and | * Rhodamine 6G is a toxic cancer linked pinkish black powder '''(MW: 479.02 g/mole)''' | ||
* Avoid all dimethyl sulfoxide and rhodamine 6G skin contact by wearing gloves and goggles | |||
* Dimethyl Sulfoxide should be kept in the flammable cabinet when not in use | * Dimethyl Sulfoxide should be kept in the flammable cabinet when not in use | ||
* When in use, dimethyl suloxide should be kept in the fume hood | * Rhodamine 6G should be capped at all times when not in use to inhibit inhalation and free floating dust particles in the air | ||
* When in use, dimethyl suloxide should be kept in the fume hood and rhodamine 6G should be used very carefully | |||
* If spilled, wipe up dimethyl sulfoxide with paper towel while wearing gloves | * If spilled, wipe up dimethyl sulfoxide with paper towel while wearing gloves | ||
* If spilled, wipe up rhodamine 6G residue first with paper towel soaked in cold water, then wipe over again with acetone while wearing gloves | |||
* Keep all spills of dimethyl sulfoxide contained in the fume hood | * Keep all spills of dimethyl sulfoxide contained in the fume hood | ||
* Wash hands after removing gloves that were in contact with dimethyl sulfoxide | * Keep all spills of rhodamine 6G in a closed space; rhodamine 6G contamination is easily transmitted through repeated glove or equipment contact | ||
* Limit the contact of rhodamine 6G on uncontaminated surfaces or equipment by changing your gloves upon first contact with rhodamine 6G | |||
* Wash hands after removing gloves that were in contact with dimethyl sulfoxide or rhodamine 6G | |||
==PVOH 146K Prepared Samples== | ==PVOH 146K Prepared Samples== |
Revision as of 22:02, 25 February 2013
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OBJECTIVES
90:10 PVOH Clay Hydrogel Preparations
50:50 PVOH Clay Hydrogel Preparations
90:10 Rhodamine 6G Dye Ratio Solution Preparation
90:10 Rhodamine 6G Dye Ratio Calculations (0.900g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0655mL M1V1 = M2V2 1μM (RG6)x 6mL = (0.0655mL)M2 M2 = 92μM (RG6) 92μM (RG6)(1x10-6M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.002g (RG6) 50:50 Rhodamine 6G Dye Ratio Solution Preparation
50:50 Rhodamine 6G Dye Ratio Calculations (0.500g PVOH) x (8/100) x (1/1.1008 g/mL (DMSO))= 0.0364mL M1V1 = M2V2 1μM (RG6)x 6mL = (0.0364mL)M2 M2 = 165μM 165μM (RG6)(1x10-6M / 1μM RG6)(0.05L)(479.02g RG6 / 1 mole RG6)= 0.004g (RG6) Important DMSO and Rhodamine 6G Safety
PVOH 146K Prepared Samples
Notes
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