User:Karlena L. Brown/Notebook/PVOH Research/2013/02/04: Difference between revisions
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* Complete freeze and thaw crosslinked clay cycle #1 | * Complete freeze and thaw crosslinked clay cycle #1 | ||
==90:10 PVOH Clay Microsphere Preparations= | ==90:10 PVOH Clay Microsphere Preparations= | ||
# | '''*Reaction should be carried out using a reflux condenser and a thermostatic water bath of 55°C.''' | ||
# Dissolve ~ 1.0g total of PVOH 146K and clay additive selected (laponite) in 5mL hot deionized H<sub>2</sub>O | |||
# | # Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub> | ||
# | # Dissolve ~ 0.6g cellulose acetate butyrate(CAB) in 25mL 1,2-dichloroethane within a round bottom flask | ||
# | # Pour the acidified PVOH 146K solution into the 25mL 1,2-dichloroethane solution within the clean round bottom flask | ||
# | # Stir 1,2-dichloroethane, CAB, and PVOH 146K solution for 30 minutes | ||
# | # After 30 minutes of stirring, add 0.75mL glutaraldehyde to the solution | ||
# | # After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 55°C | ||
# The | # Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum | ||
# Wash the collected microspheres using ~ 2-3mL of the following solvents in the following order: | |||
'''* 1,2-dichloroethane''' | |||
'''* acetone''' | |||
'''* hot water''' | |||
'''* cold water''' | |||
'''* methanol''' | |||
# Scrape off all of the collected microspheres into a small glass vial. Leave the lid off of the small glass vial. | |||
# Freeze microspheres in liquid nitrogen for ~ 2 minutes | |||
# Place on [ask Dr. Hartings name of machine again] overnight to completely dry | |||
'''* The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151]''' | |||
<br> | |||
==Important 1,2 Dichloroethane and Glutaraldehyde Safety== | |||
* Dimethyl Sulfoxide is toxic colorless liquid (78.13g / mole) | |||
* Avoid all dimethyl sulfoxide skin contact by wearing gloves and googles | |||
* Dimethyl Sulfoxide should be kept in the flammable cabinet when not in use | |||
* When in use, dimethyl suloxide should be kept in the fume hood | |||
* If spilled, wipe up dimethyl sulfoxide with paper towel while wearing gloves | |||
* Keep all spills of dimethyl sulfoxide contained in the fume hood | |||
* Wash hands after removing gloves that were in contact with dimethyl sulfoxide | |||
==Clay Exchanged Solutions & Vacuum Filtration Procedures== | ==Clay Exchanged Solutions & Vacuum Filtration Procedures== |
Revision as of 16:00, 10 February 2013
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OBJECTIVESACCIDENTALLY PLACED THIS ENTRY ON THE FEBRUARY 4th; HOWEVER, THIS ENTRY SHOULD BE UNDER FEBRUARY 6th. THANK YOU.
=90:10 PVOH Clay Microsphere Preparations*Reaction should be carried out using a reflux condenser and a thermostatic water bath of 55°C.
* 1,2-dichloroethane * acetone * hot water * cold water * methanol
* The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [1]
Important 1,2 Dichloroethane and Glutaraldehyde Safety
Clay Exchanged Solutions & Vacuum Filtration ProceduresThe clays being filtered are the clays that were prepared on 2/1/13 Setup of a vacuum filtration system: * Clean 250mL Erlenmeyer Filter Flask * Large Buchner Funnel * Whatman #42 Filter Paper * Long Secure Rubber Hose
NotesACCIDENTALLY PLACED THIS ENTRY ON THE FEBRUARY 4th; HOWEVER, THIS ENTRY SHOULD BE UNDER FEBRUARY 6th. THANK YOU.
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