OBJECTIVES
- Continue preparing polyvinyl alcohol microspheres for experimental analysis
- Dilute Rhodamine 6G dye solution down to 1μM concentration for prepared microspheres
- Prepare DSC samples for polyvinyl alcohol microspheres created
Microsphere PVOH 146K 110% CEC Laponite w/ DMHXLBR Information
Preparation of 110% Laponite Microspheres using PVOH MW 146,000-186,000
|
'
|
PVOH 146K added(g) |
0.9005
|
110% Laponite clay added (g) |
0.1002
|
Cellulose Acetate Butyrate added (g) |
0.6001
|
Glutaraldehyde added (mL) |
0.75
|
H2SO4 added (mL) |
0.25
|
Rhodamine 6G Dye Dilution Calculations
1μM Rhodamine 6G Dye Concentration
M1V1 = M2V2
1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL
DSC Analysis: Exchanged Clays & PVOH 146K Microspheres
DSC preparation and protocal involving instrumentation and usage can be found on 9/17/12 entry.
Sample Name
|
Sample Mass (mg)
|
Pan / Lid Mass (mg)
|
NaMT |
3.16 |
48.13
|
50% CEC NaMT w/ Bu3HdP+ |
2.90 |
48.91
|
110% CEC NaMT w/ DMHXLBR |
3.01 |
50.14
|
Laponite |
3.06 |
50.18
|
110% CEC Laponite w/ DMHXLBR |
3.08 |
49.52
|
90:10 PVOH 146K Laponite Dust |
2.89 |
48.87
|
90:10 PVOH 146K Laponite Medium Clumps |
2.96 |
48.89
|
90:10 PVOH 146K Laponite Large Clumps |
3.07 |
48.18
|
Notes
- After preparing the microspheres from the PVOH 146K Laponite, many of the spheres were not spherical. Large clumps of all sizes were produced rather than microspheres.
- The microspheres prepared from PVOH 146K Laponite were rigid white crystalline structures with stacked layers.
- Due to large sizes of the microspheres, they had to be ground up using a mortar and pestle before adding the 1μM rhodamine 6G dye solution.
- Also DSC was taken of the different sizes of the microspheres to determine if the structure, function, and capability of the microspheres had changed due to extensive clumping.
- When going through the process of preparing new microspheres from PVOH 146K 110% CEC Laponite w/ DMHXLBR, due to slowed vacuum filtration the sample was placed in a vial with the last solvent used for cleaning, methanol.
- When freezing the new microsphere sample in liquid nitrogen, PVOH 146K 110% CEC Laponite w/ DMHXLBR, it was frozen in a medium of methanol and vacuum filtrated to remove the methanol residue.
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