User:Karlena L. Brown/Notebook/PVOH Research/2013/02/15: Difference between revisions
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==Fluorescence Analysis: Diffusion Testing== | |||
# In a 10mL beaker containing one of the hydrogel samples with Rhodamine 6G, place ~ 4mL of H<sub>2</sub>O | |||
# Using a timer, every 15 minutes using a plastic pipette remove ~ 1-2mL of the sample | |||
# In glass cuvette marked for fluorescence, dispense the sample collected into the glass cuvette | |||
# Then place the sample filled cuvette within the sample holder to run fluorescence of the sample | |||
# Once a fluorescence reading has been run of the sample, dispose of the sample collected into a waste container | |||
# After each addition collected and disregarded of the sample, add ~ 1-2mL more H<sub>2</sub>O ensuring enough sample for the next reading | |||
# Repeat this process again for each sample over a 2 hour period | |||
==Notes== | ==Notes== |
Revision as of 20:23, 8 April 2013
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OBJECTIVES
New Method of PVOH Clay Microsphere Preparation
Reference: http://www.sciencedirect.com/science/article/pii/S0168365998000893 PVOH 146K Prepared Microsphere Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 25mL = (165μM)V2 V2 = 152.00μL
Fluorescence Analysis: Diffusion Testing
Notes
# 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
# Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu3HdP+ # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+ # Hydrogel 50:50 PVOH 146K NaMT # Hydrogel 90:10 PVOH 146K NaMT # Hydrogel control PVOH 146K # Hydrogel 90:10 PVOH 146K Laponite
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