User:Karlena L. Brown/Notebook/PVOH Research/2013/02/15

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(PVOH Film Preparations)
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==PVOH Film Preparations==
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==OBJECTIVES==
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'''Prepared PVOH films in water:'''
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* Continue to run fluorescence testing on samples containing Rhodamine 6G dye
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* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
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* Prepare new microsphere samples using new method of preparation
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'''(Actual Mass = 0.4934g)'''
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* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
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* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
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'''(Actual Mass = 0.9965g)'''
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* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
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'''Standard PVOH Film Protocol:'''
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==New Method PVOH Clay Microsphere Preparations==
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* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
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# Dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected 25mL hot deionized H<sub>2</sub>O
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* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
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# Heat solution at 100°C for ~ 12-15min
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* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
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# Cool solution for ~ 5min and then add 25mL of mineral oil to the sample
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# Place sample solution prepared in a blender to form a more homogeneous mixture / emulsion
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# After 7 minutes of stirring, add some Rhodamine 6G dye to the solution based upon the ratio selection ''' 90:10 vs. 50:50'''
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# After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
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'''Notes:'''
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'''Reference:''' http://www.sciencedirect.com/science/article/pii/S0168365998000893
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* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
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* Heat was reduced when solutions began to boil too rapidly
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* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
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==PVOH 146K Prepared Samples & Dye Preparations==
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'''1μM Rhodamine 6G Dye Concentration'''
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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  1μM (RG6)x 25mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 271.74μL
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'''1μM Rhodamine 6G Dye Concentration'''
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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  1μM (RG6)x 25mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 152.00μL
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
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| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
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|-
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| 50:50||110% CEC NaMT w/ DMHXLBR||0.50760||0.49130||25||165||152
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|-
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| 90:10||110% CEC NaMT w/ DMHXLBR||0.90990||0.10450||25||92||272
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|-
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| 90:10||110% CEC Laponite w/ DMHXLBR||.90100||0.09980||25||92||272
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|}
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==Notes==
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* Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H<sub>2</sub>O added in order for fluorescent analysis include:
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      # 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR
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      # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR
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      # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR
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      # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
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* For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels
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* Fluorescent samples run and completed for 2hr diffusion rate determinations:
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      # Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>
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      # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>
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      # Hydrogel 50:50 PVOH 146K NaMT
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      # Hydrogel 90:10 PVOH 146K NaMT
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      # Hydrogel control PVOH 146K
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      # Hydrogel 90:10 PVOH 146K Laponite
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Revision as of 01:31, 18 February 2013

PVOH Research Main project page
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OBJECTIVES

  • Continue to run fluorescence testing on samples containing Rhodamine 6G dye
  • Prepare new microsphere samples using new method of preparation

New Method PVOH Clay Microsphere Preparations

  1. Dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected 25mL hot deionized H2O
  2. Heat solution at 100°C for ~ 12-15min
  3. Cool solution for ~ 5min and then add 25mL of mineral oil to the sample
  4. Place sample solution prepared in a blender to form a more homogeneous mixture / emulsion
  5. After 7 minutes of stirring, add some Rhodamine 6G dye to the solution based upon the ratio selection 90:10 vs. 50:50
  6. After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days

Reference: http://www.sciencedirect.com/science/article/pii/S0168365998000893

PVOH 146K Prepared Samples & Dye Preparations

1μM Rhodamine 6G Dye Concentration

  M1V1 = M2V2
  1μM (RG6)x 25mL = (92μM)V2    V2 = 271.74μL

1μM Rhodamine 6G Dye Concentration

  M1V1 = M2V2
  1μM (RG6)x 25mL = (165μM)V2    V2 = 152.00μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.507600.4913025165152
90:10110% CEC NaMT w/ DMHXLBR0.909900.104502592272
90:10110% CEC Laponite w/ DMHXLBR.901000.099802592272

Notes

  • Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H2O added in order for fluorescent analysis include:
     # 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR
     # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR
     # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR
     # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
  • For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels
  • Fluorescent samples run and completed for 2hr diffusion rate determinations:
     # Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
     # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
     # Hydrogel 50:50 PVOH 146K NaMT
     # Hydrogel 90:10 PVOH 146K NaMT
     # Hydrogel control PVOH 146K 
     # Hydrogel 90:10 PVOH 146K Laponite


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