User:Karlena L. Brown/Notebook/PVOH Research/2013/02/15

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(PVOH Film Preparations)
(New Method PVOH Clay Microsphere Preparations)
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# Cool solution for ~ 5min and then add 25mL of mineral oil to the sample
# Cool solution for ~ 5min and then add 25mL of mineral oil to the sample
# Place sample solution prepared in a blender to form a more homogeneous mixture / emulsion
# Place sample solution prepared in a blender to form a more homogeneous mixture / emulsion
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# After 7 minutes of stirring, add some Rhodamine 6G dye to the solution based upon the ratio selection ''' 90:10 vs. 50:50'''
+
# After 7 minutes of stirring, add some Rhodamine 6G dye to the solution based upon the ratio selection ''' (90:10 vs. 50:50)'''
# After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
# After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days

Revision as of 02:32, 18 February 2013

PVOH Research Main project page
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OBJECTIVES

  • Continue to run fluorescence testing on samples containing Rhodamine 6G dye
  • Prepare new microsphere samples using new method of preparation

New Method PVOH Clay Microsphere Preparations

  1. Dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected 25mL hot deionized H2O
  2. Heat solution at 100°C for ~ 12-15min
  3. Cool solution for ~ 5min and then add 25mL of mineral oil to the sample
  4. Place sample solution prepared in a blender to form a more homogeneous mixture / emulsion
  5. After 7 minutes of stirring, add some Rhodamine 6G dye to the solution based upon the ratio selection (90:10 vs. 50:50)
  6. After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days

Reference: http://www.sciencedirect.com/science/article/pii/S0168365998000893

PVOH 146K Prepared Samples & Dye Preparations

1μM Rhodamine 6G Dye Concentration

  M1V1 = M2V2
  1μM (RG6)x 25mL = (92μM)V2    V2 = 271.74μL

1μM Rhodamine 6G Dye Concentration

  M1V1 = M2V2
  1μM (RG6)x 25mL = (165μM)V2    V2 = 152.00μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.507600.4913025165152
90:10110% CEC NaMT w/ DMHXLBR0.909900.104502592272
90:10110% CEC Laponite w/ DMHXLBR.901000.099802592272

Notes

  • Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H2O added in order for fluorescent analysis include:
     # 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR
     # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR
     # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR
     # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
  • For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels
  • Fluorescent samples run and completed for 2hr diffusion rate determinations:
     # Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
     # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
     # Hydrogel 50:50 PVOH 146K NaMT
     # Hydrogel 90:10 PVOH 146K NaMT
     # Hydrogel control PVOH 146K 
     # Hydrogel 90:10 PVOH 146K Laponite


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