User:Karlena L. Brown/Notebook/PVOH Research/2013/02/15

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(PVOH 146K Prepared Samples & Dye Preparations)
(PVOH 146K Prepared Samples & Dye Preparations)
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==PVOH 146K Prepared Samples & Dye Preparations==
==PVOH 146K Prepared Microsphere Samples & Dye Preparations==
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
   M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
   M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>

Revision as of 01:24, 26 February 2013

PVOH Research Main project page
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  • Continue to run fluorescence testing on samples containing Rhodamine 6G dye
  • Prepare new microsphere samples using new method of preparation

New Method of PVOH Clay Microsphere Preparation

  1. In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H2O
  2. Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample
  3. Cool solution for ~ 5 minutes, then remove the stir bar, and add PVOH clay sample to a blender
  4. Afterwards, then add 25mL of mineral oil to the sample in the blender
  5. Blend sample solution prepared in blender for ~ 7 minutes on high to form a more homogeneous mixture / emulsion (creating a suspension of microspheres)
  6. After 7 minutes, quicklya dd some Rhodamine 6G dye to the solution based upon the ratio selection (90:10 vs. 50:50)
  7. After the addition of the dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
  8. Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours


PVOH 146K Prepared Microsphere Samples & Dye Preparations

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 25mL = (92μM)V2    V2 = 271.74μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 25mL = (165μM)V2    V2 = 152.00μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.507600.4913025165152
90:10110% CEC NaMT w/ DMHXLBR0.909900.104502592272
90:10110% CEC Laponite w/ DMHXLBR.901000.099802592272


  • Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H2O added in order for fluorescent analysis include:
  # 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR
  # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR
  # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR
  # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
  • For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels
  • Fluorescent samples run and completed for 2hr diffusion rate determinations:
  # Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
  # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
  # Hydrogel 50:50 PVOH 146K NaMT
  # Hydrogel 90:10 PVOH 146K NaMT
  # Hydrogel control PVOH 146K 
  # Hydrogel 90:10 PVOH 146K Laponite
  • In regards to the spectra, all samples maintained a quick diffusion rate indicating that dye crosslinking during the freeze / thaw cycle is pertinent when making hydrogels because it slows down the diffusion rate
  • For the hydrogels in which the dye was added, but not crosslinked into the material, their diffusion rate was the quickest -- a significant amount of dye leaked out of those hydrogels throughout the 2 hour period
  • Comparing the 50:50 vs. the 90:10 ratio of PVOH / clay, the hydrogels with 50:50 ratio had more dye leak out than the 90:10 ratio hydrogels indicating a more effective pressure stimuli

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