User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20

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(Expanded Method of PVOH Clay Microsphere Preparation)
(Hydrogel Pressure Samples Tested)
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==Hydrogel Pressure Samples Tested==
==Hydrogel Pressure Samples Tested==
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| align="center" style="background:#f0f0f0;"|'''90:10 PVA MW 146,000-186,000:110% Lamponite hydrogel pressure tests'''
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| align="center" style="background:#f0f0f0;"|'''90:10 PVOH MW 146K 110% CEC Lamponite w/ DMHXLBR Hydrogel'''
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Revision as of 02:58, 25 February 2013

PVOH Research Main project page
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OBJECTIVES

  • Prepare more microspheres samples expanding upon new previous method developed
  • Begin fluorescence detection through pressure testing analysis of hydrogel samples

Expanded Method of PVOH Clay Microsphere Preparation

  1. In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H2O
  2. Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample
  3. Cool solution for ~ 5 minutes, then remove the stir bar, and add PVOH clay sample to a blender.
  4. Afterwards, then add 35mL rather than 25mL of mineral oil to the sample in the blender.
  5. Blend sample solution prepared in blender for ~ 7 minutes on high to form a more homogeneous mixture / emulsion (creating a suspension of microspheres)
  6. After 7 minutes, quickly pour solution into several mini 20mL vials and then add some Rhodamine 6G dye to the solution based upon the ratio selection (90:10 vs. 50:50)
  7. Next, quickly freeze the PVOH clay sample immersed in safflower oil in liquid nitrogen for ~ 5 min. The vial should be held in the liquid nitrogen until the sample is completely frozen throughout.
  8. After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
  9. Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours

Hydrogel Pressure Testing Protocol

  1. Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions.
  2. Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample.
  3. Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
  4. Once placing the sample in a Pasteur pipette, attach a ribber bulb to the top of the pipette, and allow 3mL of distilled H2O enter into the pipette by squeezing ribber bulb
  5. Progressively squeeze the bulb in order to expel the 3mL of H2O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching)
  6. Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis

Hydrogel Pressure Samples Tested

90:10 PVOH MW 146K 110% CEC Lamponite w/ DMHXLBR Hydrogel '
Pasteur Pipette ShapeAmount of Hydrogel Used(g)
Pasteur pipette w/ no modifications0.1575
Pipette stem bent at slight angle < less than 90 degrees0.1134
Pipette stem bent at slight angle < 90 degrees beginning stem of pipette0.1397
Pipette w/ three pockets at top0.1039
Pipette bent twice in the stem0.1158
Pipette bent at top ≈ 90 degree angle w/ pipette sides almost touching0.1108
Pipette with a twist in middle0.0993
Pipette bent twice in the middle0.1416
Pipette w/ two pockets in the top0.1041
Pipette w/ top bent & sides almost touching = 90 degree angle0.1258
Plain Pasteur pipette w/ no modifications: Run #20.1210

Notes


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