User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample. | # Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample. | ||
# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | # Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | ||
# Once placing the sample in a Pasteur pipette, attach a | # Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing ribber bulb | ||
# Progressively squeeze the bulb in order to expel the 3mL of H<sub>2</sub>O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching) | # Progressively squeeze the bulb in order to expel the 3mL of H<sub>2</sub>O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching) | ||
# Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis | # Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis |
Revision as of 00:11, 25 February 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
PVOH 146K Prepared Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL Hydrogel Pressure Testing Protocol
Hydrogel Pressure Samples Tested
Notes |