User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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==Notes== | ==Notes== | ||
* Previously prepared microspheres on 2/15/13, formed layer of film at the bottom of each beaker not sole microspheres. The microspheres were actually encompassed into the film | |||
* Originally the type of oil used to prepare microspheres previously was mineral oil; however, safflower oil was used this time as substitute | |||
* Safflower oil has a higher freezing point than mineral oil | |||
* In case samples placed in 10mL vials after being removed from blender begin to settle, sonicate each sample before freezing it in liquid nitrogen -- keeping microspheres immersed in organic layer | |||
* For the 90:10 PVOH 146K 110% CEC Laponite w/ DMHXLBR, 45mL vs. 35mL of safflower oil was used | |||
* Rhodamine 6G dye maintains high affinity for organics; therefore, Rhodamine 6G dye will be added later to each microsphere newly prepared | |||
* DMSO dye prepared hydrogel samples did not leak dye at a quick rate versus dyes prepared in H<sub>2</sub>O | |||
* To analyze fluorescence and execute pressure test, hydrogel samples had to be cut into small pieces and smashed up against the glass in order to emulate sheer pressure tests | |||
* To simulate sheer pressure testing on the hydrogels, Pasteur pipettes were bent at different angles to determine which formation would provide the most sheer pressure and largest fluorescence signal. (Pipette pictures soon to be uploaded) | |||
* The same sample, 90:10 PVOH 146K 110% Laponite w/ DMHXLBR, was used to determine which Pasteur pipette modification proved useful and most result worthy when providing a fluorescence signal. | |||
* It appears as if using an unmodified pipette or a pipette with a bend at the top of the pipette so the sides are almost touching produced the maximum amount of sheer pressure. | |||
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Revision as of 00:47, 25 February 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
PVOH 146K Prepared Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Hydrogel Pressure Testing Protocol
Hydrogel Pressure Samples Tested
Notes
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