User:Karlena L. Brown/Notebook/PVOH Research/2013/02/22: Difference between revisions

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(Autocreate 2013/02/22 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
 
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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Prepare more microsphere samples expanding upon new previous method developed
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Continue fluorescence detection through pressure testing analysis of hydrogel samples
'''(Actual Mass = 0.4934g)'''
* Run DSC sample analysis on dry clay samples previously prepared on 2/08/13
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==PVOH 146K Prepared Microsphere Samples & Dye Preparations 3==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.  
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
 
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
|-
| 50:50||Laponite||0.50220||0.50250||25||165||61
|-
| 50:50||110% CEC Laponite w/ DMHXLBR||0.50960||0.50460||25||165||61
|-
| 50:50||NaMT||.50250||0.49980||25||92||109
|}
 
==Notes==


'''Notes:'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
* Heat was reduced when solutions began to boil too rapidly
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)




Revision as of 22:56, 25 February 2013

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed
  • Continue fluorescence detection through pressure testing analysis of hydrogel samples
  • Run DSC sample analysis on dry clay samples previously prepared on 2/08/13

PVOH 146K Prepared Microsphere Samples & Dye Preparations 3

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 Laponite 0.50220 0.50250 25 165 61
50:50 110% CEC Laponite w/ DMHXLBR 0.50960 0.50460 25 165 61
50:50 NaMT .50250 0.49980 25 92 109

Notes


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