User:Karlena L. Brown/Notebook/PVOH Research/2013/02/27

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OBJECTIVES

  1. Continue fluorescence detection through pressure testing analysis of hydrogel samples (new modifications & better results)

Hydrogel Pressure Samples Tested

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1084
2 90:10 130K NaMT 0.1044
3 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.1092
4 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1050
5 50:50 130K NaMT 0.1050
6 90:10 130K Laponite 0.1015
7 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1080
8 50:50 130K Laponite 0.1036
9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
10 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036
11 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
12 90:10 146K Laponite 0.1026
13 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1052
14 50:50 146K Laponite 0.1045
15 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1145

Notes

  • The pipettes that provided the best fluorescence results for each sample were both the straight plastic pipette and 90° angle bent pipette.
  • All samples run maintained a composition incorporating Rhodamine 6G dye
  • All samples on 2/27/13 were tested using the bent pipette containing the 90° angle. (See figure listed below)

Figure 1. Image of bent pipette used for pressure testing


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