User:Karlena L. Brown/Notebook/PVOH Research/2013/03/08

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(Hydrogel Pressure Samples Tested 3)
(Hydrogel Pressure Testing Protocol (Straight Pippette))
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==OBJECTIVES==
==OBJECTIVES==
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==Hydrogel Pressure Testing Protocol (Straight Pippette)==
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# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample
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# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
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# Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing rubber bulb
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# Progressively squeeze the bulb in order to expel the 3mL of H<sub>2</sub>O and apply a pressure to the hydrogels – dispensing  Rhodamine 6G dye (dye leaching)
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# Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis
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==Microspheres: Separation & Vacuum Filtration Procedures==
==Microspheres: Separation & Vacuum Filtration Procedures==

Revision as of 14:28, 24 March 2013

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OBJECTIVES

Microspheres: Separation & Vacuum Filtration Procedures

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