User:Karmella Haynes/BME494 ProjectTemplate: Difference between revisions

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==Background==
==Overview & Purpose==
[[Image:CirEmblemHandwriteLogo2.png|thumb|140px||left|Text describing the image]]
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<!-- Background information on the natural Lac operon. This should be based on Group Presentation 2 -->
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==Design: Our genetic circuit==
==Design of a New Device==


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'''OUR GENE SWITCH''': 
<!-- Show a network/ circuit diagram of your device. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) -->
 
<!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) -->


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==Building: Assembly Scheme==
==Building the New Device==


<!-- Illustrate and describe how you will build your lac switch. Incorporate information from Group Presentation 2 -->
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'''Synthetic DNA Layout'''


<!-- Show each DNA fragment in the order in which you propose to assemble them into a single plasmid. This can be a series of connected blocks. You do not have to show the plasmid as a circle. -->


'''Resources'''
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==Testing: Modeling and GFP Imaging==
==Testing the New Device==


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==Our Team==
==About the Designer==
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[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
</div>
<br>
<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px">
[[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']]
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
</div>
<br>




<br><br><br>
<br><br><br>


==Works Cited==
==References==
[1] Full reference.
[1] Full reference.


Revision as of 11:35, 14 April 2014


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Overview & Purpose

Text describing the image












Overview & Purpose

Text describing the image














Design of a New Device

Text describing the image












Building the New Device

Synthetic DNA Layout


Resources
















Testing the New Device


A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.


AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.


COLLECTING EMPIRICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.



Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.











Human Practices

Danger of Chemicals in Farmlands














About the Designer

Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.





References

[1] Full reference.

[2] Full reference.

[3] Full reference.


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