User:Karmella Haynes/Notebook/ASU courses/2012/10/24

10/24/12

• Check results for section 1
• Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes

Check results (spot check)

• Run 5 μL (1/20 sample) on a 1% gel (etBr)
• Expected size = ~200 - 300
• Marker: [1]

Group 1 1. ctrl 2. ctrl 3. plasmid 4. plasmid 5. plasmid 6. none 7. none 8. none Group 2 1. ctrl 2. ctrl 3. none 4. none 5. none 6. plasmid 7. plasmid 8. plasmid

Prep for Week 2, Wednesday (Sections 2, 3)

• Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
• Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)

• Master Mixes
  
  • DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
  • To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)

DNA/primer mixes: Make ~1/3 "patients" positive for marker

Per Section!