User:Karmella Haynes/Notebook/ASU courses/2012/10/24: Difference between revisions
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==10/24/12== | ==10/24/12== | ||
* Check results | * Check results for section 1 | ||
* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes | * Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes | ||
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'''Check results''' (spot check) | '''Check results''' (spot check) | ||
* Run 5 μL (1/20 sample) on a 1% gel (etBr) | |||
* Expected size = ~200 - 300 | |||
* Marker: [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg] | |||
{| class="wikitable" | {| class="wikitable" width=500 | ||
|- valign="top" | |- valign="top" | ||
| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid | | Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid | ||
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DNA/primer mixes: Make ~1/3 "patients" positive for marker | DNA/primer mixes: Make ~1/3 "patients" positive for marker | ||
{| | {| {{table}} | ||
|- | |- | ||
| Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s''' | | Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s''' | ||
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Per Section! | Per Section! | ||
{| | {| {{table}} | ||
| Reagent || (+) || Mix+2 (x55) || (-) || Mix+2 (x85) | | Reagent || (+) || Mix+2 (x55) || (-) || Mix+2 (x85) | ||
|- | |- |
Revision as of 16:16, 2 January 2013
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10/24/12
Check results (spot check)
Prep for Week 2, Wednesday (Sections 2, 3)
DNA/primer mixes: Make ~1/3 "patients" positive for marker
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