User:Karmella Haynes/Notebook/ASU courses/2012/10/24 - Revision history

Karmella Haynes at 23:16, 2 January 2013

2013-01-02T23:16:00Z

←Older revision		Revision as of 23:16, 2 January 2013
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	DNA/primer mixes: Make ~1/3 "patients" positive for marker		DNA/primer mixes: Make ~1/3 "patients" positive for marker

-	{\| ~~class="wikitable" border=1~~	+	{\| {{table}}
	\|-		\|-
	\| Section \|\| Groups \|\| Total Patients \|\| Pos ctrls \|\| Pos patients x3 reps \|\| '''TOTAL (+)'s''' \|\| Neg ctrls \|\| Neg patients x3 reps \|\| '''TOTAL (-)'s'''		\| Section \|\| Groups \|\| Total Patients \|\| Pos ctrls \|\| Pos patients x3 reps \|\| '''TOTAL (+)'s''' \|\| Neg ctrls \|\| Neg patients x3 reps \|\| '''TOTAL (-)'s'''
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	Per Section!		Per Section!
-	{\|	+	{\| {{table}}
	\| Reagent \|\| (+) \|\| Mix+2 (x55) \|\| (-) \|\| Mix+2 (x85)		\| Reagent \|\| (+) \|\| Mix+2 (x55) \|\| (-) \|\| Mix+2 (x85)
	\|-		\|-

2012-10-25T08:54:36Z

10/24/12

←Older revision		Revision as of 08:54, 25 October 2012
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	==10/24/12==		==10/24/12==
-	* Check results ~~ofr~~ section 1	+	* Check results for section 1
	* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes		* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes

2012-10-25T07:53:55Z

10/24/12

←Older revision		Revision as of 07:53, 25 October 2012
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	'''Check results''' (spot check)		'''Check results''' (spot check)
	* Run 5 μL (1/20 sample) on a 1% gel (etBr)		* Run 5 μL (1/20 sample) on a 1% gel (etBr)
		+	* Expected size = ~200 - 300
	* Marker: [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]		* Marker: [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]

2012-10-25T07:52:14Z

10/24/12

←Older revision		Revision as of 07:52, 25 October 2012
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	'''Check results''' (spot check)		'''Check results''' (spot check)
		+	* Run 5 μL (1/20 sample) on a 1% gel (etBr)
		+	* Marker: [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]

	{\| class="wikitable" width=500		{\| class="wikitable" width=500

2012-10-25T07:49:25Z

10/24/12

←Older revision		Revision as of 07:49, 25 October 2012
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	'''Check results''' (spot check)		'''Check results''' (spot check)

-	{\| class="wikitable"	+	{\| class="wikitable" width=500
	\|- valign="top"		\|- valign="top"
	\| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid		\| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid

2012-10-25T07:38:38Z

10/24/12

←Older revision		Revision as of 07:38, 25 October 2012
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	\|- valign="top"		\|- valign="top"
	\| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid		\| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid
-	\| [[Image:KAH102412_gel1.~~tiff~~\|250px]]	+	\| [[Image:KAH102412_gel1.png\|250px]]
	\|}		\|}

2012-10-25T07:32:39Z

10/24/12

←Older revision		Revision as of 07:32, 25 October 2012
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	{\| class="wikitable"		{\| class="wikitable"
-	\|-	+	\|- valign="top"
		+	\| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid
		+	\| [[Image:KAH102412_gel1.tiff\|250px]]
	\|}		\|}

2012-10-25T07:27:55Z

10/24/12

←Older revision		Revision as of 07:27, 25 October 2012
Line 8:		Line 8:

	==10/24/12==		==10/24/12==
		+	* Check results ofr section 1
	* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes		* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes
		+
		+
		+	----
		+
		+	'''Check results''' (spot check)
		+
		+	{\| class="wikitable"
		+	\|-
		+	\|}
		+

2012-10-25T02:21:46Z

10/24/12

←Older revision		Revision as of 02:21, 25 October 2012
Line 8:		Line 8:

	==10/24/12==		==10/24/12==
-	* ~~To-do list~~	+	* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes

2012-10-25T02:20:22Z

10/24/12

←Older revision		Revision as of 02:20, 25 October 2012
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	==10/24/12==		==10/24/12==
	* To-do list		* To-do list
		+

	----		----

-	'''Prep for Week 2, Wednesday (~~Section 1~~)'''	+	'''Prep for Week 2, Wednesday (Sections 2, 3)'''

	* Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)		* Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
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	\| Section \|\| Groups \|\| Total Patients \|\| Pos ctrls \|\| Pos patients x3 reps \|\| '''TOTAL (+)'s''' \|\| Neg ctrls \|\| Neg patients x3 reps \|\| '''TOTAL (-)'s'''		\| Section \|\| Groups \|\| Total Patients \|\| Pos ctrls \|\| Pos patients x3 reps \|\| '''TOTAL (+)'s''' \|\| Neg ctrls \|\| Neg patients x3 reps \|\| '''TOTAL (-)'s'''
	\|-		\|-
-	\| Thurs 9:30 \|\| 17 \|\| 34 \|\| 17 \|\| 36 \|\| '''53''' \|\| 17 \|\| 66 \|\| '''83'''	+	\| Thurs 9:30 am \|\| 17 \|\| 34 \|\| 17 \|\| 36 \|\| '''53''' \|\| 17 \|\| 66 \|\| '''83'''
		+	\|-
		+	\| Thurs 1:30 pm \|\| 17 \|\| 34 \|\| 17 \|\| 36 \|\| '''53''' \|\| 17 \|\| 66 \|\| '''83'''
	\|}		\|}