User:Karmella Haynes/Notebook/ASU courses/2015/09/30: Difference between revisions
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* Banana demo: transformations | * Outreach - Banana demo: transformations | ||
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'''Banana scent demo: | '''Banana scent demo: transformations''' | ||
* Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell) | * Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell) | ||
* Amp resistant | * Amp resistant | ||
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* Transform with 0.5 uL DNA in 10 uL dH<sub>2</sub>O, or just water (neg) | * Transform with 0.5 uL DNA in 10 uL dH<sub>2</sub>O, or just water (neg) | ||
* Use 50 μL chem. competent cells for each | |||
* Plate on 100 μg/mL ampicillin | |||
* Grow o/n at 37°C to get colonies | |||
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Revision as of 18:19, 30 September 2015
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Banana scent demo: transformations
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