Project Description
Creation of parts & assembly of constructs for all projects
- Polycomb Project
- Test Gal4-ATF's: built ✓, expression test ✓, functional test ✓
- Polycomb-ATF's (with myc tag): built ✓, stable lines ✓
- Histone methylation reporters: built ✓
- Neo translation induction test constructs: built ✓, functional test ✓ (done, but uncertain outcome)
- Gal4-PEST-EED neo-inducible expression constructs: built ✓, functional test (failed)
- Full-length histone methylation sensors (+/- DPRE, constitutive Gal4-EED): built ✓, expression test (in progress)
- Polycomb-ATF no-VP64 controls: built ✓, stable lines ✓
- ES cell expression constructs: cloning into pSB31 in progress
- miR Sensor Project
- RFP reporter/ repressors: built ✓, expression test ✓
- CFP reporters: built ✓, expression test ✓
- 9 pair-wise combos of RFP/CFP: built ✓, expression test ✓
- 0, 5, 10x UAS site Target constructs: Francesca
- miR-34 Repressor constructs: Francesca
- New pcDNA3.1+/ΔCMV,hygro & pcDNA3.1+/ΔCMV,zeo vectors: Andrew
- miR-34 sensor tests: Daniel
- Fly miR Sensor Project
- Basic parts: in progress
- Transient transfections of BioBricked fluors: pending
Notes
- This project is: perpetual
- My status: stilllllll cloning.
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