User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/24

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12/24/09

  • ✓ Minipreps: KAH121-125/MV2 (1 ea.), KAH93 (2), KAH120/MV2 re-picks (4)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
MV2 = 4529
1. KAH121/MV2 = 8102
2. KAH122/MV2 = 8174
3. KAH123/MV2 = 8002
4. KAH124/MV2 = 8174
5. KAH125/MV2 = 8246
6. KAH93 = 4839
7-10. KAH120/MV2 = 7930 		E/P digest 12/24/09
15 μL/lane; 1% agarose
DNA(plasmid) 3.0
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ ~15 min.

--> 3/5 low yield plasmids (KAH121-125) failed. Try re-growing from streaks later.
--> KAH93/V0120 failed. Try re-transformation.


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