User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/30

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12/30/09

  • ✓ Minipreps: re-picks KAH137, 138 (6 each)
  • ✓ Cultures: KAH117(2), 118(2), 119(4); KAH94, 95/JDS43 (6 each)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
 E/P digest 12/30/09
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> None worked. Streptavidin tag seems to be difficult to clone with. Unsure why. Ask Joe.



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