The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
 Karmella's BioBrick Cloning
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
11/30/12
- Assembly: CMVTetO2:K (KAH3)/ MV; expression vector for Vi's mammalian cell expression experiment
Assemblies
- KAH3/MV2: (1) KAH3/(X/P)/751 + (2) MV2/(X/P)/4529
> Digests (Fermentas FD)
| Reagent |
Volume |
|
30 μL/lane, 1% agarose; Ladder
|
| DNA (plasmid) |
up to 25 μL
|
| 10x buffer |
3.0
|
| enzyme 1 |
1.0
|
| enzyme 2 |
1.0
|
| dH2O |
---
|
| |
30 μL --> 37°C/ ~15 min.
|
> Measure conc.'s of gel purified fragments
| Sample |
OD260 |
260/280 |
ng/μL
|
| 1. KAH3 (X/P) |
0.028 |
1.96 |
28.41
|
| 2. MV2 (S/P) |
0.085 |
1.93 |
85.02
|
> Ligations
| Ligation |
Plate results (lig : neg crtl) 11/31/12
|
| 1. KAH3 (X/P)/751, 8 ng + MV2 (S/P)/4529, 25 ng |
KAH3/MV2 #:1 (Pick #)
|
| 2. MV2 (S/P)/ 25 ng |
|
| |
1 |
2 |
|
| Insert DNA |
0.3 |
--- |
|
| Vector DNA |
0.3 |
0.3 |
|
| 2x lgn buf (Roche) |
5.0 |
5.0 |
|
| T4 ligase (NEB) |
1.0 |
1.0 |
|
| dH2O |
3.4 |
3.7 |
|
| |
10 μL |
10 μL |
|
|
Karmella's BioBrick Cloning
