User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/03: Difference between revisions
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== | ==12/03/12== | ||
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* | * Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | ||
---- | ---- | ||
'''Assemblies''' | '''Assemblies''' | ||
# | # KAH111/C-His: KAH111/(E/P)/1123 + C-His/(E/P)/3600 | ||
* Digests (Fermentas FD) | * Digests (Fermentas FD) | ||
** | ** Already done by Behzad | ||
* Measure conc.'s | * Measure conc.'s | ||
** Behzad: KAH111 = 15 ng/μL | |||
** Behzad: C-His = 57 ng/μL | |||
* | |||
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| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | ||
|- | |- | ||
| 1. | | 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng || <font color="blue">new BioBrick #:1 (Pick #)</font> | ||
|- | |- | ||
| 2. | | 2. C-His(E/P)/ 25 ng || | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 | | || 1 || 2 | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || 1.0 || --- | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || 0.5 || 0.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 2.5 || 3.5 | ||
|- | |- | ||
| || | | || 10 μL || 10 μL | ||
|} | |} | ||
Revision as of 19:40, 3 December 2012
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12/03/12
Assemblies
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